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New breakthrough in wastewater monitoring

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Introduction

Researchers from Bangor University have optimised a method to evaluate the risk of infectious viruses in wastewater, providing a crucial tool for protecting public health. Read the full report published here in MDPI.

Traditional techniques for measuring viruses in wastewater already tell us genetic information but don’t indicate whether the viruses are still potentially infectious to humans. The newly developed method uses a special dye, propidium monoazide (PMAxx), in a test that is called capsid integrity-RT-qPCR (ci-RT-qPCR).

The team, led by Dr Jessica Kevill and Prof Davey Jones, carried out extensive tests on live viruses, heat-inactivated viruses, and viruses present in wastewater. The study looked at all viruses commonly found in wastewater, including adenovirus, hepatitis A, influenza A virus, and norovirus.

Full citation

Kevill JL, Farkas K, Ridding N, Woodhall N, Malham SK, Jones DL. Use of Capsid Integrity-qPCR for Detecting Viral Capsid Integrity in Wastewater. Viruses. 2024; 16(1):40. https://doi.org/10.3390/v16010040

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